Plant Preservative Mixture (PPM™)

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What is Plant Preservative Mixture?

Plant Preservative Mixture (PPM™), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination.

Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

The Benefits of Plant Preservative Mixture

At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
PPM™ does not affect the genetic profile of the plants.

How To Use Plant Preservative Mixture?

Follow the given steps to prepare the tissue culture media involving PPM™:
Take a beaker and add 800 ml of sterile distilled water to it.
To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
Add water to make 950 ml of the volume of the media.
Adjust the pH of the media between 5.8-6.0.
Add 8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
Make up the volume of the media to 1000ml.
Autoclave the medium for 20 minutes at 15 psi and 121℃.

Your media is ready!

Product Name	Plant Preservative Mixture (PPM™)
Feature	Autoclavable and heat stable
Use	Preservative for plant tissue culture media
pH	5 - 7
Capacity	Available in different volumes of 30mL, 100mL, 250mL, 500mL, and 1L.
Shape	Cylindrical container
Material	Liquid
Color	Clear to light amber
Odor	Mild
Storage Temp	39° F (4° C).
HTS Code	3808999000

Document:

Video Tutorials

Video Demonstration

How Effective is PPM™? (PPM™ Demonstration)

What is Plant Preservative Mixture?

Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

The Benefits of Plant Preservative Mixture

At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
PPM™ does not affect the genetic profile of the plants.

How To Use Plant Preservative Mixture?

Follow the given steps to prepare the tissue culture media involving PPM™:
Take a beaker and add 800 ml of sterile distilled water to it.
To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
Add water to make 950 ml of the volume of the media.
Adjust the pH of the media between 5.8-6.0.
Add 8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
Make up the volume of the media to 1000ml.
Autoclave the medium for 20 minutes at 15 psi and 121℃.

Your media is ready!

Product Name	Plant Preservative Mixture (PPM™)
Feature	Autoclavable and heat stable
Use	Preservative for plant tissue culture media
pH	5 - 7
Capacity	Available in different volumes of 30mL, 100mL, 250mL, 500mL, and 1L.
Shape	Cylindrical container
Material	Liquid
Color	Clear to light amber
Odor	Mild
Storage Temp	39° F (4° C).
HTS Code	3808999000

Document:

Video Tutorials

Video Demonstration

How Effective is PPM™? (PPM™ Demonstration)

View full details

Contributor	Plant Name	Contaminant ID	PPM concentration (mL/L)	Tissue Type/Application	Result / Comments
Dr. Ashish Dave	Malus rootstock	Fungi and Bacterial	1ml/L	added to media	PPM @1ml/L added to Malus rootstocks shoot multiplication media. It was very effective in controlling bacteria, without causing any phytotoxicity and growth aberrations.
Richard P	Hemp	Fungi and Bacterial	3% PPM	Explants	We wash explants 3% PPM in sterile water for surface sterilization and 3% PPM in MS Media for shoot initiation
Yaroslov Fuglevich	Paulownia	Bacteria and fungus	1ml/L	Use PPM in the culture media, added before sterilization	very effective, does not cause phytotoxicity
Arthur P	Philodendron Pink Princess	Fungi and Bacterial	1 mL/L	Explants	40mL/L & 0.5mL/L philodendron pink princess Explants were washed in 4% PPM and were inoculated on media with 0.05% PPM no infections .
Matt Miller	Venus Flytrap	Fungi and Bacterial	1 mL/L	Explants and Seeds	Put PPM in media pre-autoclave. Dramatically reduces contamination on both seeds and explants.
Patrick ****	Ceropegia Genus	Bacteria and Fungi	2 mL/L	Stem Explant	Added to media prior to autoclaving. Prevents contamination.
Safa Labidi	Potato	Bacteria	1ml/L	Shoot/Callus	used 1ml/l in our culture media to avoid contaminants
Md Faruk Hossain	Aloe Vera	Rhizome	2 mL/L		Prior to autoclave
Natalia Martinez-Ochoa	Tobacco	Fungal and bacterial	2 mL/L	Leaf midvein and callus tissue culture. PPM is added to tissue culture media when it is being prepared, before or after autoclaving	Very Effective in preventing contamination
Hasnorita Abdul Rahman	Pineapples	Fungal	2 mL/L	Sucker	Put onto medium, very effective to reduce contamination rate
Dr. Kamlesh Patel	Lonicera Species	Bacteria	1 mL/L	Actively Growing Shoot Tips	Very Effective in preventing and eliminating bacteria in tissue culture
Yengl Gukh	Bean	Fungus	100ml purchased	leaf	Incredible fungal removal effects were seen in callus cultures
Jennie	Coconut	bacterial	0.1 ml/L	embryo	0.1ml/L PPM were incorporated into tissue culture media
Mareid Glediee	Yuzus	Bacteria	2 ml/L	Shoot	It was used for disinfection when preparing sections in tissue culture
MARIA LOURDES VICENTE	Pepper	bacteria	50ml purchased	anthers	surface sterilization, in media
Petolescu Ceraselaz	Rubus fruticosus	bacteria and fungus	2 mg/L	I use PPM in the culture media, added before sterilization;	very effective
Eliseo Tesón	Drosera,Nepenthes,Dionaea	bacteria and fungal	2 mL/L	Leaf pullings and Seed sowing	I do use PPM when other disinfection procedures do not work, for example leaf pullings in drosera. Also I do use it when I exchange flasks with other people that already used PPM as a preservative i do add PPM if i have to replace the seedlings. Also a rinse of PPM after sterilizing Nepenthes seed with NaDCC has proben to be very effective to kill the remaining bacterial contamination.
Innan Godínez García	Vanila planifolia (Vainilla) Orquídea	bacteria, yeast, and fungi	1 mL/L	319/5000 The explants were washed with a Tween 20 1ml / L solution for 20 min outside the laminar flow hood. Inside the hood they were subjected to a 10% chlorine solution for 20 min and 2 drops of Tween 20 in 100ml of water. Then they were left in PPM for 10min. and were planted in MS 1/2 medium with 0.5 ml / L of PPM apical meristem and node	very effective
Carlos Urrutia	Orchid		1 mL/L	apply it after sterilization
Jesus Lucina Romero	Blueberry	bacterial	0.5 mL/L	Shoot	Add 0.5ml/L before sterile de medium
Daniel Zavala Ortiz	Tetrastigma		2 mL/L	suspension culture	Preventing contamination during bioreactor cultures
Jeanmarie Molina	Taxus Globosa S		0.5 mL/L	stem, root	Preventing contamination during bioreactor cultures;
Remi Bonnart	Vitis (Grape)	endophytic bacteria	15 mL/L	Nodal Segments;	Helpful to control endophytic bacteria in Vitis shoots taken from potted plants and introduced to tissue culture for several weeks with minimal/no toxic effects
Hail Z. Rihan	Cauliflower		0.5 mL/L	Cauliflower microshoots	The use of 0.5 mL L−1 of PPM with culture medium was found to be effective in controlling contamination and keeping the growth capacity of microshoots.
Matilde E. Uribe	Traubia modesta	Bacterial	1 mL/L	Bulbs of T. modesta(Roots, the upper-third of the stem, and dry outer scales were removed)	Bacterial contamination could be efficiently controlled with streptomycin and PPM
Dawood Ahmad	Curcuma, Kaempferia and Zingiber	Endogenous Contamination	2 mL/L	Buds	Preservative mixture (PPM) treatment which helped to minimize the endogenous contamination.
Junji Miyazaki	Petunia hybrida		5 mL/L	Upper young axillary buds	1. HPLC revealed that axillary buds from upper young nodes absorbed significantly more PPM compared with those from the lower nodes. 2. Indexation indicated successful eradication of bacterial contaminants from upper young axillary buds after vacuum-infiltration with 5 ml l−1 PPM
Sergei Krasnyanski	Cotton		1 mL/L	Cotton seeds	Plant preservative mixture rinses were also included in the sterilization procedures to improve the efficiency of tested protocols.
Pedro Sansberro	Ilex paraguariensis		3.5 mL/L	Nodal segment explants of Ilex paraguariensis
Timothy A. Rinehart	Hydrangea macrophylla (Thunb.) Ser. and Hydrangea paniculata		2 mL/L	Hydrangea seed	0.5× Gamborgs solid media in conjunction with Plant Preservative Mixture (PPM), and by sterilizing seed with trichloro-s-triazinetrione (Trichlor). Assays of physiology were conducted by sterilizing seed.
Junji Miyazaki	Black kangaroo paw		5 mL/L (under vacuum)	Infected axillary buds and basal-stem calli	Indexation of plantlets raised from PPM-treated tissues indicated successful eradication of the endophyte from basal-stem calli, and from shoots regenerated from them.
Freddy Mora	T. modesta	Bacterial, Fungal	1 mL/L	The bulbs of T. modesta	The contamination was only by fungi because the bacterial contamination could be efficiently controlled with streptomycin and PPM as biocides.
Gustavo Zapata	Carnivorous Plants (Dionaea)	Fungus	1 mL/L	Leaf	prepare media with MS
Mike ****	Ficus carica		2 mL/L	Shoots	Media supplement and explant disinfestation
Gemplant b.v.b.a	Cortaderia		1-2 ml/L	add to medium before autoclavation
Omobolanle Ade-Ademilua	Peperomia pelucida	Endophytic bacteria, Fungi	40mL/L and 0.75mL/L	Leaf	40mL/L was used in disinfecting explants and 0.75mL/L added to media (solid)
Jorn Hansson	Gerbera, orchids, perennials		1mL/L	Shoots	mixed in media right after sterilization
Issaac Reyes	Cephalotus follicularis	Fungus, Bacteria	80mL/L	Seeds	80ml/l, Soak seeds in 0.8ml ppm/10ml water+1/3MS media, FREE PGR'S, Sucrose, No pH Adjust for 3-4 hrs. Desinfection for cephalotus follicualris seeds
Margaret Clark	Native hawaiian seeds		1mL/L	Seeds	To blotter paper in petri dishes
Dr. Kamlesh R. Patel	Lonicera	Bacterial	1mL/L	Shoot	Added to the culture medium
Kun Wei	Norway Spruce	Bacterial	0.5 mL/L	Somatic Embryos	Prevent the possible infections from handling.
Xinlu Chen	Hornwort	Fungus	1 mL/L	leaves	controlling fungi
Katrina Dlugosch	Centaurea spp.		1 mL/L (1% by volume)	Seeds	Germination of seeds
Oleg Bosyy	Vaccinium		1.5 mL/L	Shoots	addition of PPM to the medium
Issaac ******	Dionaea Muscipula	Fungus, Bacteria	2 mL/L	Leaf	Add 2ml/l in MS media and sterilize.
Dr. Raquel Folgado	Aloe		0.05 - 0.1 mL/L	Shoots
Dr. Raquel Folgado	Magnolia		0.05 - 0.1 mL/L	Shoots
Dr. Raquel Folgado	Avocado		0.05 - 0.1 mL/L	Shoots
Dr. Chunsheng Lu	Berries		0.5 - 5ml/L	Shoot Tip
Manmeet Singh	Potato		1.2ml/L	Stems
Martha Orozco	Asperagus		10 mL/L
Martha Orozco	Tomato		10 mL/L
Martha Orozco	Rice		10 mL/L
Osagie Idehen	Fern		1 mL/L
Osagie Idehen	Sweet potato		1 mL/L
Jayakumar Pon Samuel	Maize		1mL/L
Jayakumar Pon Samuel	Soybean		1 mL/L
Jayakumar Pon Samuel	Canola		1mL/L
Mr. Micah Stevens	Juglans		2 mL/L	Shoots
Dr. Jiping Zhao	Petunia		2 mL/L	Leaf
Dr. Qingzhen Jiang	Alfalfa		3 – 5 mL/L	Leaf
Dr. Qingzhen Jiang	Medicago		3 – 5 mL/L	Leaf
Dr. Qingzhen Jiang	Switchgrain		3 – 5 mL/L	Leaf
Chetty Venkaterwen	Rice		3.5 mL/L	Seed/Embryo
Chetty Venkaterwen	Ciban		2 mL/L	Leaf/Shoot
Chetty Venkaterwen	Tomato		2.5 mL/L	Seedlings
Kaitlin J. Palla	Clusia		5 mL/L
Kaitlin J. Palla	Kalanchoe		5 mL/L
Kaitlin J. Palla	Poplar		5mL/L
Jim Bo	Orchids		0.3 mL/L	Nodes	Growing orchids
RUANGWIT PORRUAN	Nepenthes		1-2 mL/L	Shoot	Add 1-2 mL/L PPM to media before sterilization.
Annie Lachapelle	Rosa hybrida		10 mL/L	Shoot	Mix with culture
Quoirin M.	Bambusa	Fungi and bacteria	3 ml/L	shoot segment	Desinfestation for introduction in vitro
armin mustafic	orchid		1 mL/L	leaf	Great, five star product!
Dr. Kamlesh R. Patel	Hosta		1 mL/L	bulb	Liquid Culture
Naoaki Okamoto	Nepenthes		5ml/L	seed	In vitro culture of Nepenthes
Marc Martinez	Sarracenia	Bacterial	1 mL/L	seed	Bacterial and fungal prevention for in vitro multiplication.
William Conner	Citrus		4 mL/L	Shoot / Tip	eliminates fungus, does not seem to have effect on plant
Michelle Steenberghen	Cortaderia		1.5ml/l	shoots	add before autoclavation
Choo ********	OIL		40mL/L & 0.5mL/L	SEED	Explants were washed in 4% PPM and were inoculated on media with 0.05% PPM
Erin *****	Canola	fungal	1 mL/L	callus, shoot	to be added to tissue culture media
Dr. Kamlesh R. patel	Elm	Bacterial	1 mL/L	shhot tips	In agar solidified medium.
Hoang Tuan Vu	Wasabi		1 mL/L	Shoot	Plant preservertive mixture
Deborah McCown	Amelanchier	Bacteria and Fungi	0.5 ml/L	Shoot Culture	Tissue Culture media
Maria Consue Medina	blueberry	Bacterial	2 mL/l	shoots	before autoclave in the culture media
Carolina Rugeles	Ornamentals	Bacteria, Fungus	2 mL/l	Tissue Culture	Prepare in the Media solution
Natalia Campistany	cucumber	Endogenus bacteria	1 mL/L	plant	mixed in the solid media
Obaid ur Rahman	banana	Fungal, bacterial	1 mL/L	shoot tip cultures	PPM added at a concentration of 1 ml/L in liquid culture media and sterilized for 5 mins and 121 degrees centigrade
Meenakshi Santra	Wheat		0.5ml/l	wheat embryo culture	we use in regeneration media and sometimes to kill the fungi in regenerated plants
Desiree Ho	Cantharanthus		0.01 mL/L	Nodes and shoot meritsem	Preventing microbial contamination in tissue culture media.
Joanna *****	maize	Aspergillus	2 mg/l	embryo	Soke overnight
Chan Woei Yee	Anubias	Bacterial	1 mL/L	Corm	Plant Maintenance
Bruno La Rosa	Nepenthes	Fungi spores, bacteria	1 mL/L	Seed	1 mL applied to 1 L of MS Basal medium mixture with sucrose and BAP.
Joshua Spece	Hosta		1-2 mL/L	meristem bud	PPM used in the media. Greatly improves contamination rate.
Julie *****	raspberry		1 mL/L	shoot	use on contaminated cultures that we must continue to multiply
Maria Consue Medina	Blueberry	Bacterial	2.5 mL/L	Shoots	Before autoclave
Gerardus VanDerMeij	Impatiens	Bacterial	1 mL/L	Shoots	Used in propagation to keep cultures clean
Viji ******	Orchids		1 mL/L	Shoot	Media preparation and cleaning tissues
Md Faruk Hossain	Aloe Vera	Rhizome	2 mL/L		Prior to autoclave
Leong MY	Mango	Leaf	0.2 mL/L	Cloning
	Paulownia	Bacterial and Fungus	1 mL/L	In the culture media and prior to sterilization	Very effective, does not cause phytotoxicity

Good for long term storage of explants.

I have a professional setup so i don't have problems with contamination, so i don't use PPM all the time. I do back up strains for long term storage and use it for that. I feel safe that if some contamination does find its way into the vessel, that it will not grow.

Much less Contamination

I use 1.5mL of PPM per liter of MS media for Ribes propagation and have much less contamination issues than without. I use it for all now.

great tissue culture preservative

We are happy with the advantages that we see when using PPM. It definitely has improved our tissue culture work.

Liquid gold!

I love this stuff! I don't know what I would do without it! Thank you for providing a high quality product at a good price!

PPM

I am a beginner hobbyist in Plant tissue culture and this PPM has made life so much easier, which a greatly decreased contamination rate. Really quick shipping and super helpful when Ive had questions.

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Plant Preservative Mixture (PPM™)