Introducing PCT's Brand New Plant Growth Regulators!

8th Jul 2021

Introducing PCT's Brand New Plant Growth Regulators!


We have got good news!

Plant Cell Technology has extended its catalog of products to assist plant growers of all different kinds! This time, we brought you all plant growth regulators (PGRs) or hormones used in tissue culture. And, if you are confused about the products and their uses, then this article is only for you. In this article, we talk about different plant growth hormones and their applications in tissue culture, why are they required, and much more!

What are plant growth regulators?

Plant growth regulators are small chemical molecules produced by plants for their growth and development. They are also known as phytohormones. Plant hormones are categorized into two groups based on their origin: natural if produced within plants and synthetic hormones if produced in laboratories. Majorly there are five classes of plant growth hormones: Auxin, Cytokinin, Gibberellin, Ethylene, and Abscisic acid. But, practically during plant tissue culture we mainly use auxin, cytokinins, and gibberellins for the proper development of plants. So, in this article, we will only look at these three hormones that are also called plant growth promoters.

Preference Center


Cytokinins are the growth hormones that promote cell division in plant roots and shoots. Naturally occurring cytokinins are only purine-derived compounds. But there are also some synthetic purine-derived cytokinins which include kinetin, zeatin, and 6-benzylaminopurine. There are other synthetic phenylurea derived cytokinins which include diphenylurea and thidiazuron. Of the naturally occurring cytokinins, zeatin and 2iP (2-isopentenyl adenine) have some use in plant tissue culture.

The use of naturally occurring cytokinin is now widespread because they are expensive and unstable. So, the best alternatives to these hormones include benzylaminopurine, kinetin, and metatopolin. Let’s learn more about these plant hormones.

1a. Benzylaminopurine (BAP)

What is it?

It’s a synthetic cytokinin, a plant growth regulator or plant hormone that supports the development of plants when added to growth media.


  • It influences plant growth and development, sets blossoms, and stimulates fruit richness by stimulating cell division.
  • It modifies MS media for shoot initiation in cultures
  • It stimulates seed germination.
  • It’s an adenine-based cytokinin that’s perfect to be supplemented with culture media including MS media, Gamborg’s media, and Chu’s N6 media.

1b. Kinetin

What is it?

It’s a synthetic cytokinin that stimulates cell division when added to growth media such as Murashige and Skoog media in conjunction with auxins.


  • It helps to stimulate cell division and promote the proper growth of plants.
  • It induces callus development and shoots initiation from callus at its later stages.
  • It’s used for shoot induction and proliferation.

1c. Metatopolin

What is it?

It’s an adenine-based cytokinin required for the shoot regeneration of cultures.


  • It’s used for shoot regeneration.
  • Plants supplemented with meta topolin cause lesser root inhibition compared to other cytokinins.


Auxins are a class of growth hormones that promote both cell division and cell elongation. These are categorized into two categories based on their origin: natural if derived from plants and synthetic if produced in labs. The naturally occurring auxin includes IAA (indole-3-acetic acid). Some other auxins include IBA (indole-3-butyric acid), 2,4-Dichlorophenoxyacetic acid (2,4-D), and 1-naphthylacetic acid (NAA). Plant Cell Technology brought you two of these commonly used hormones that include IAA and IBA. Let’s learn more about them.

2a. Indole-3-Acetic Acid (IAA)

What is it?

It’s a natural auxin, a plant growth regulator or plant hormone that is required for the proper growth and development of plants.


  • It has a role in cell enlargement and division, tissue differentiation, and response to light and gravity.
  • In the plant tissue culture, it’s used for the organ development of plants like shoots and roots.
  • It’s used as a supplement in growth media like MS media or Gamborg’s media for callus initiation.

2b. Indole-3-Butyric Acid (IBA)

What is it?

It’s an auxin family plant hormone that is used in agriculture and tissue culture for the development of plant roots.


  • It helps to initiate adventitious root formation in cultured tissues.
  • It helps to stimulate callus development.
  • It’s thought to be a precursor of indole acetic acid (IAA).


What is it?

It’s a growth hormone and plant growth regulator that promotes the proper growth and development of plants in culture.


  • It has a role in cell division and elongation of tissues in cultures.
  • In some plants including Arabidopsis and peas, it helps in seed germination.
  • It’s supplemented with growth media like Murashige and Skoog media for the proper development of young leaves and stems.
  • It’s essential for the flowering of plants.
  • It’s also used for agricultural and home gardening uses.


That’s not it! Plant Cell Technology brought two more essential elements of your tissue culture media that help to keep your culture healthy and promote proper development.

3a. Folic Acid

What is it?

It’s a supplement having a role in the growth and development of cultured plants. It’s also known as folate or B9 Vitamin.


  • Folic acid helps to fulfill the deficiency of folates in crops. Any disturbance in a plant's folate metabolism leads to severe growth inhibition and low productivity.
  • It has an essential role in amino acid metabolism and nucleic acid synthesis in plants.
  • They can be supplemented to staple crops like rice, potato, and maize that contain insufficient amounts of folates.

3b. Myo-Inositol

What is it?

It’s a sugar-like carbohydrate that is important for the normal growth and development of plants.


  • It helps in the cell wall formation of plant cells.
  • It protects the plants against salt stress.
  • It’s involved in the storage and transportation of some plant hormones like auxin.

You got what you wanted! Plant Cell Technology is helping you in every way it can. So, do share your experience with us on the given email ID, and don’t forget to subscribe to PCT newsletters to get a regular update on our products, blogs, and videos. Yes, we are consistently making YouTube videos to educate you on tissue culture processes so please let us know how you feel about it! And finally, keep shopping for your tissue culture products from your favorite PCT Store.

Happy culturing!!




Anjali Got some PCT story to share?
We would love to hear your feedback and suggestions!
Selected PCT product stories will get featured on our website as well. Not to forget, some goodies might find a way to your home along with it.
Share your suggestions & story with me at

Our Flagship Product

PPM Shop Now
Featured Articles

Tissue Culture Propagation of Banana

Banana is a tropical fruit that is consumed by individuals in raw and cooked forms. It is believed to have originated in Southeastern Asia, in countries like India, Philippines, Malaysia, etc. The edi …

read more

How PPM™ Can Save Your Tissue Culture Experiment

Plant Preservative Mixture (PPM™) is a robust formulation used as a broad-spectrum biocide in plant tissue culture experiments. By targeting bacteria, fungi, and other contaminations …

read more

PPM vs Antibiotics - A Comparison

Whether you are a seed to fruit kinda grower, or a plant cloning guru, you know how vital it is to keep your plants free from contaminants. From airborne microbial infections, airborne microbial …

read more

Tissue Culture Contamination and 7 Easy Steps of Prevention

Again, contamination! Tissue culture is a long and laborious process and it feels vexing when fungus or bacteria attack our lovely cultures. Culturing cells in the labs requires a lot of …

read more