Plant Cell Technology
Find the answers to those frequently asked questions
Plant Cell Technology Products and Services FAQs
General Tissue Culture FAQs
How can I use the starter kit?
The starter kit contains the basic materials you need to get started in tissue cultures, such as MS Medium, Agar, PPM™, and Culture vessel. It’s perfect for introducing your plant tissues in a lab environment.
Visit this link to learn how to use this product in your tissue culture applications.
How can I save on the shipping cost of the products?
You can contact our distributor close to you with your requirements. It will help you cut down on the shipping costs of the products. Visit this link to locate your nearest distributor.
How do I establish an account with your company?
You can create an account with us via our website and register here.
If you would prefer to place orders directly with our fulfillment team rather than use our website, you can email our team directly: email@example.com.
What is the shelf life of PPM™ when stored at 4 C?
The shelf life of PPM™ is 18 months refrigerated. Feel free to search “Dahila” in our Customer Usage Table and our blog articles for any information!
What is Biocoupler™?
Biocoupler™is a simplified temporary immersion bioreactor (TIB). It couples (connects) two glass jars (70/450 finish) with an integrated filter to facilitate a smooth medium flow between the two jars. Its unique adjustable vane allows control over liquid flow, helps in preventing the passage of small propagules, and reduces air-lock.
It also contains a microporous vent filter that allows pressure equalization to atmospheric pressure, helping prevent contamination. The device is more efficient space-wise and time-wise, compared to other currently available TIB systems. Further, the Biocoupler™ not only can be used by hobbyists but also by large-scale plant businesses.
What is Biocoupler™ used for?
Biocoupler™ is used for the multiplication of your tissue culture plants. It can also be utilized as a seed sprouting and or rooting. The Biocoupler™ is a simplified temporary immersion bioreactor. Think tissue culture meets hydroponics.
It’s affordable, requires less shelf space, is efficient, and is easy to use. You can use it to produce hundreds and thousands of plants for your plant business efficiently.
How many times do I need to tilt Biocoupler™ in a day?
You need to tilt the Biocoupler™ once a day or week based on the plant species you’re growing. Remember it’s not time-dependent and you can do it at any time of the day. Also, keep your plants immersed in the medium for at least 30-60 seconds. Just the number of tilting the Biocoupler™ is important.
How can I book a consultation call with a tissue culture expert?
To schedule a consultation, visit this link, choose your desired date and time, enter personal details, pay for the consultation, and your call will be scheduled!
You will receive both an email and a message regarding the confirmation of your consultation call.
If you need any help with scheduling your call, reach out to us at firstname.lastname@example.org.
Does Plant Cell Technology offer protocols to introduce plants in tissue culture?
Our lab director shares his protocols for the plant he works with in the lab, such as Monstera, Philodendrons, Anthurium, nepenthes, etc. on our Youtube channel.
You can also find some protocols in our blogs, that we create referring to the research papers published in high-quality journals.
Other than these, we do not offer or sell any protocols in written format.
I need suggestions to start my tissue culture lab.
Whether you need help with starting your own tissue culture lab or getting out of the stuck phase of the tissue culture process, we would recommend you take our consultation services to directly have a video chat with a tissue culture expert.
I need help with my orders.
For any inquiry or help related to your orders, reach out to email@example.com and our team will help you best with your queries.
How can I learn more about the upcoming master class?
Would I need to bring something to the class with me?
Not at all! You just need to come up with your essentials, and we provide you with everything you need to perform tissue culture experiments during class. We provide you with handouts for reference, chemicals to learn hands-on, and equipment that you will require during your classes.
Come empty-handed and leave with a plethora of tissue culture knowledge!
Do you offer lab-building services?
Yes! We do help culturists to build and establish their tissue culture lab. However, before that, we will need to talk with you regarding your lab requirements, expectations, and plans.
To get our help in building your lab, email us at firstname.lastname@example.org.
Can I become a Brand Ambassador for PCT?
Yes! But, you must qualify for the program and be approved by our marketing team. To review the qualifications and apply, visit our ambassador’s page here.
What are the basic materials I need to get started in tissue culture?
You will need MS medium (or substitute), gelling agent (agar or gellan gum), and sugar to establish any plant in tissue culture. Use the Plant Preservative Mixture (PPM) in your media to avoid contamination.
Where can I buy my tissue culture requirements?
You can buy MS media, agar, gellan gum, PPM™, Plant Hormones, and culture vessels from your one-stop PCT store.
How can I prevent contamination in tissue culture?
You will need to follow general tissue culture best practices to prevent contamination in tissue culture, such as:
- Wear a PPE kit, such as gloves and masks. (If you’re working under open flames, avoid wearing gloves).
- Work under a laminar flow hood
- Clean your culture area using alcohol
- Sterilize all chemicals, tools, and equipment used in the process to prevent contamination
- Clean your hands before working with plants
- Use healthy and disease-free plants as a source of plant tissue
- Use PPM to give your plants all-round protection against all types of contamination
How to use MS medium?
Follow the given steps to use MS media:
- Take a beaker and add 800 ml of sterile distilled water to it.
- To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
- Add water to make 950 ml of the volume of the media.
- Adjust the pH of the media between 5.8-6.0.
- Add PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
- Make up the volume of the media to 1000ml.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
What are the uses of tissue culture?
Tissue culture has many applications:
- Produce plants at a commercial scale rapidly
- Produce disease-free plants
- Produce plants irrespective of their seasons
- Produce haploid and hybrid plants
- Conserve plant species
- Produce a large number of plants in a small space
Explore more about the applications and advantages of tissue culture in our blog: https://www.plantcelltechnology.com/blog/
Can I build a tissue culture lab at home?
Yes, you can create a home-based tissue culture lab. For that, you need at least one room with all basic equipment, tools, and chemicals. Clean the room thoroughly and ensure it’s not crowded with other materials.
Contamination may be a major issue in a home-based lab, that’s why we recommend following all basic protocols to maintain aseptic conditions, such as wearing a PPE kit and using Plant preservative mixture (PPM™) in your media.
How much money do I need to build a tissue culture lab?
To get started, you need at least $5k to build a basic tissue culture lab. However, it’s suitable for hobbyists or small-scale culturists. So, for an advanced lab, where you can produce plants on large scale, you need at least $25K to get started. This can go up based on the space you choose to build your lab, and the equipment, tools, and chemicals you equip your lab with to get into the tissue culture process.
Where can I find a procedure or protocol for my desired tissue culture plant?
You can search on search engines, such as google and yahoo, with the keywords “tissue culture protocol for [insert your plant name]” or “[insert your plant name] tissue culture”.
You can find plenty of research papers containing protocols for the plant.
What do I need to do to maintain aseptic conditions in my tissue culture lab?
Just follow these general instructions:
- Wear appropriate protective clothing, such as a lab coat, gloves, and a face mask. These will help to prevent any contamination from your body and ensure the safety of the operator.
- Use a laminar flow hood or a clean bench to work under sterile conditions. This will help to minimize the risk of airborne contaminants entering the working area.
- Sterilize all equipment and materials before use. Autoclave glassware, media, and other materials, and wipe down surfaces with a suitable disinfectant.
- Keep the tissue culture lab clean and organized. This will help to prevent the accumulation of dust and other contaminants and ensure that you can easily find the equipment and materials you need.
What are the factors that affect plant tissue culture success rates?
The factors that affect plant tissue culture success rates include:
- Explant type
- Culture medium
- Growth regulators
- Physical environment
- Contamination control
What is the role of plant growth regulators in tissue culture?
Plant growth regulators, also known as plant hormones, play a crucial role in tissue culture. They control the growth and differentiation of cells and tissues in vitro and are used to induce cell division, regulate morphogenesis, and promote organogenesis in plant tissues.
What is the role of auxin in plant tissue culture?
- Auxin promotes cell division and elongation in plant tissues.
- It induces rooting in explants and helps to establish root systems in tissue culture.
- Auxin stimulates callus formation, which can be used to generate large amounts of undifferentiated cells for tissue culture.
- It is involved in the formation of somatic embryos in tissue culture.
What are some auxins used in plant tissue culture?
The auxins used in tissue culture applications include 3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D). The type of hormone and the concentration to be used in the process depends on the plant species you work on.
What is the role of cytokinin in plant tissue culture?
- Cytokinin is a plant growth regulator that stimulates cell division and differentiation in plant tissues.
- It is commonly used in tissue culture to promote shoot formation and branching.
- Cytokinin can be used in combination with auxin to promote callus formation and somatic embryogenesis.
What type of cytokinins are used in plant tissue culture?
The auxins used in tissue culture applications include kinetin, zeatin, benzylaminopurine (BAP), and meta-topolin (mT). The specific type and concentration of cytokinin used will depend on the tissue type and desired response in tissue culture.
Where can I sell tissue culture plants?
Tissue culture plants can be sold to various markets such as nurseries, gardening centers, and horticulture wholesalers. You can also sell them online through e-commerce platforms or directly to individual customers via social media or a website. It's important to research and identify potential markets and understand their requirements before selling tissue culture plants.