Gellan Gum

What is Gellan Gum?

Solidifying agents play a crucial role in tissue culture media by providing the necessary support to hold cells, tissues, or explants in place, ensuring they receive optimal nutrients for growth and development.

Gellan gum, an anionic polysaccharide, is employed as a replacement for agar in various clinical bacteriological and plant tissue culture media. Its structural composition comprises a repeating unit of tetrasaccharide, including two D-glucose residues, one L-rhamnose residue, and one D-glucuronic acid residue.

It serves as an excellent alternative for nutrient media in plant tissue culture, yielding a clearer and more transparent gel than agar. Additionally, it’s required in only half the concentration of agar typically used in tissue culture media.

The Benefits of Gellan Gum

• Easy to use
• Acts as a solid support for the growth and development of explants
• Allows the absorption of nutrients by the plant cells
• The transparency of the gel allows one to clearly see the development of roots or contamination from the start.
• Cost-effective

How To Use Gellan Gum?

Follow the given steps to prepare the tissue culture media using MS media:

• Take a beaker and add 800 ml of sterile distilled water to it.
• To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
• Add water to make 950 ml of the volume of the media.
• Adjust the pH of the media between 5.8-6.0.
• Add 4 grams of PCT’s Gellan Gum to the media and keep stirring.
• Make up the volume of the media to 1000ml.
• Autoclave the medium for 20 minutes at 15 psi and 121℃.

Your media is ready!

Documents

• COA
• MSDS

Gellan Gum is used as microbial solid culturing media. The substance is often used in doses ranging between 0.2% (2g/L) to 0.4% (4g/L) (w/v) to solidify plant tissue culture media. PPM can be mixed and autoclaved when creating Gellan Gum media.