What is Kinetin?
Kinetin is a synthetic cytokinin that stimulates cell division when added to growth media such as Murashige and Skoog media in conjunction with auxins.
Feature Highlights of Kinetin
Molecular Formula : C10H9N5O
Storage: 4 ℃
Shelf Life: 60 Months
Assay: min. 99%
Sulphated Ash: max. 0.05%
Spectral Ratio (A250nm/A260nm): ~0.60
Spectral Ratio (A280nm/A260nm): ~1.27
Spectral Ratio (A290nm/A260nm): ~0.73
Suitability for Tissue Culture: Passes
The Benefits of Kinetin
Easy to use
Helps to stimulate cell division and promote the proper growth of plants.
Use to supplement tissue culture media, such as MS media
Induces callus development and shoots initiation from the callus at its later stage
Used for shoot induction and proliferation
How To Use Kinetin?
Follow the given steps to prepare the tissue culture media using PCT’s supreme grade agar:
Prepare liquid tissue culture media using PCT’s MS media and add sugar based on the quantity of media you’re making (generally for 1-liter media 25-30 grams of sugar is added), 1ml PPM, and 4 grams of gellan gum.
Then, add the desired quantity of the plant hormone.
Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
Want to learn more about PCT’s Kinetin? Visit www.plantcelltechnology.com/blog/.