Loading... Please wait...

PCT Blog

The Importance of Plant Tissue Culture to Industrial Growing

Posted

Biological studies of plants have been gaining momentum over the years, fueled by a few specific areas of research. One of these areas is the steadily developing world of plant tissue culture.

What is Plant Tissue Culture?

Even though the average Joe may have never heard of plant tissue culture, it has been gaining popularity for decades - and if you have ever purchased produce grown in mass quantities, then you have probably digested some plants or fruits produced using plant tissue culture.

Plant tissue culture is a method used to grow new plants using tissue or cells from an existing plant, much the same as cloning. Originally said to have been developed for cultivators growing delicate orchids, plant tissue culture is now used throughout the blossoming cannabis community.

For small scale growers as well as large scale producers, the tissue culture method is urging the already surging cannabis industry, and it looks set to continue moving the cannabis industry forward with stable momentum. This is especially evident on large scale productions where the integrity and quality of the harvest are critical.

What's more, the plant tissue culture umbrellas several methods that can be used to cultivate and maintain new plant tissues or cells. The key to this master plan, however, lies in perfectly calibrated growing conditions. The environment should be sterile and kept under stringent requirements to make sure it remains free of contaminants and viruses. In addition to maintaining this environment, growers must ensure that plants receive the correct nutrients and PPM to keep them healthy.

So, we understand what tissue culture process is, now let's look at why its becoming more and more important in large scale production…

Use of Plant Tissue Culture

While there are plenty of advancements in the biological community that provide little by way of practical application, plant tissue culture certainly makes its stand in the practical world. 

Tissue culture is used to produce platelets from cells or tissues, which are then used for the following:

Cloning

Clones are cells that have come from asexual reproduction or a parent cell. By using the plant tissue culture process, these clones can be produced in quick succession and with uniformity - making it simpler for producers to supply consistent quality products on-demand. By making effective use of PPM and plant tissue culture, large scale productions can grow high yields to meet market standards and demands. In the cannabis industry, this is rapidly becoming an essential feature in many cultivation operations.

Somaclonal Variation

Sometimes, plant cells of a particular culture will have discrepancies. These differences are known as "somaclonal variation" and are used as an umbrella term to refer to genetic differences in plants that come from the same plant culture. Cultivators and researchers often use somaclonal variation to propagate specific varieties of plant species.

Transgenic Plants

When genetic engineering is used to move a gene from one organism into another, that gene is referred to as a transgene. When an organism has one of these transferred genes, then the organism is referred to as a transgenic organism. Cells that have had transgenes placed into them can be used to generate platelets, and these platelets can then grow into plants that are often sought after because of their transgenic qualities.

Resistance to Weedicides

When cultivating using the plant tissue culture process, weedicides are sometimes added in the beginning stages, and only in minute concentrations, so that a resistance to weedicides can be fostered in the new cells. The weedicide dosage is usually increased or decreased systematically until the cultures contain the correct dosage and, therefore, have developed a resistance. Once the resistance is determined in a cell, these cells would then be used to generate plants and plantlets.

Plant Tissue Culture: Advantage in Industrial Growing

You may be wondering how the above information makes the tissue culture process vital to industrial growing. 

Well, all of the uses referenced above serve the purpose of helping growers develop new plants that are free from airborne microbial contamination and diseases from contaminated water. Additionally, the plants can be regenerated rapidly, and on the industrial level, there are two keys to success: perfect (consistent) quality and speed of production to meet industrial demands.

Several methods fit into the tissue culture process, but none are more prominent in plant and cannabis tissue culture than micropropagation.

For cultivators to grow cannabis that will sell and supply the industry, it is imperative that the cannabis plant culture is free of harmful chemicals and contains specific genes and characteristics.

By using micropropagation, clones of the cannabis plant will offer the following benefits:

  • Identical characteristics. If a supplier needs a specific ratio of THC to CBD or a particular strain to supply for medicinal use, then micropropagation can be used to achieve this with precision.
  • Speed. Speed of production is often an essential ingredient for any business - whether trading stocks or growing plants. Micropropagation cuts down the growing time and speeds up the entire production process without sacrificing quality and integrity.
  • No seeds or cross-pollination. Because micropropagation is based on clones, no seeds or cross-pollination is required.
  • Health. Plants can be engineered to have resistance to certain viruses, diseases, and even weedicides.

There are many benefits to micropropagation and plant biotechnology in general. Companies like Plant Cell Tech understand this and offer quality products for growers to start making use of tissue culture, cell technology, and DIY tissue culture today.

The cannabis industry is teetering on the brink of a mass industrial scale, and applied plant biotechnology is critical for how the industry surges forward. For the cannabis sector, it seems as though cannabis plant culture and cannabis tissue culture is one way to meet the stringent quality requirements on an industrial scale without sacrificing genetic integrity or plant quality.

Outside Resources:

https://www.cell.com/trends/biotechnology/fulltext...

https://www.intechopen.com/books/recent-advances-i...

https://www.sciencedirect.com/science/article/abs/pii/0167779987900357

https://www.sciencedirect.com/topics/biochemistry-...

View Comments


The History of Tissue Culture

Thanks to tissue culture, we are able to understand plant morphogenesis and see how single cells can differentiate and perform specific functions. This makes tissue culture studies and research an indispensable advancement, and has made many plant cultivators happy growers.By making use of the tissue culture process, growers are able to produce high-quality plants in [...]

Read More »


​How our Agar Compares to the Competition

What is Agar?Agar is a polysaccharide obtained from seaweeds and is most commonly used as a solidifying agent in plant tissue culture media. Some growers use Phytagel in place of Agar as the gelling medium in a tissue culture operation. Which one is better depends on the details of your specific application. Agar is best [...]

Read More »


What is the Tissue Culture Process and Why is it in Demand?

Tissue culture is a technique of growing new plant tissue by transferring them into an artificial environment in which they can continue to grow, and function. This article deals with tissue culture: the process, its importance, and its advantages over other forms of planting.As in most industries, profit is king in the cannabis trade. Even [...]

Read More »


PPM vs Antibiotics - A Comparison

Image Source: https://www.royalqueenseeds.com/blog-marijuana-will-be-an-antibiotic-for-the-future-n366Whether you are a seed to fruit kinda grower, or a plant cloning guru, you know how vital it is to keep your plants free from contaminants. From airborne microbial infections, airborne microbial contamination, or even waterborne pollution, cell technology has been chasing down a sustainable, long-term answer to these agricultural issues. It seems [...]

Read More »


PPM eliminates bacteria...no fungal contamination

Baby hemp plants are growing strong. No fungal contamination detected. Traces of bacterial contamination were eliminated by the PPM mixed into the gel. Plants look healthy and are growing quickly. Stay tuned for more updates.Feel free to reach out directly for more information on the concentrations used, inquiries about PPM, and partnerships.

Read More »


No contamination after 15 days --- no laminar flow hood

15 days after the tissue was placed into the jars with PPM we have no contamination

Read More »


Optimal PPM concentrations determined in order to clone Hemp plants without contamination

Francisco begins the experiment by testing various concentrations of PPM against a control (the red colored gel has no PPM in the media). Each colored gel represents a different concentration of PPM.In order to successfully sterilize the meristematic tissue we need to:1. Determine the optimal concentration of PPM needed2. Calculate the amount of time that [...]

Read More »


PCT to clone Hemp using PPM without a laminar flow hood

PCT is excited to partner with Ironside Hemp to develop an efficient in vitro propagation procedure for hemp clones. Our plants will be grown in a cost-effective laboratory without a laminar flow hood. Our technician will be using various doses of PPM to prevent contamination and maintain sterile cultures.Stay tuned as we will be sharing [...]

Read More »





To buy PPM, navigate to the PPM Store or Search for PPM in the Product Search Bar in the top left page corner

Recent Updates

Connect with us: Facebook LinkedIn Twitter