Using PPM to Determine Sterilization Rates During Tissue Culture

Posted by Zaire Williams on 1st Jun 2020

Using PPM to Determine Sterilization Rates During Tissue Culture

Francisco Palacios:

"On the first round of experiments, I used 4% PPM (10 ml. 9.6 ml of sterilize water and 0.4 ml of PPM) for 4 hours. The results weren't great, with only 4 out of the 20 plants surviving - a majority were killed during the sterilization process. While the results may look negative, I'm actually pretty happy with the results. 

Yes, some of the plants were killed, but of the ones that survived no contamination was present! That means I just need to reduce the sterilization time until I found the "sweet spot." It's important to note that these plants are very sensitive, so it's normal to run tests to determine the right times and dosages for sterilization.

For those plants that did get contaminated, I'm going to try the same percentage of PPM and time, but I'm going to make sure my explants are fully clean with no soil or anything that may cause contamination problems."

List of Plants Used In Experiment:

Pinguicula Emarginata x Gigantea
Pinguicula Florian
Pinguicula Emarginata x Hemiepiphytica
Pinguicula Weser
Pinguicula Debbertiana
Pinguicula Rotundifolia
Pinguicula Seductora
Pinguicula Cyclosecta
Pinguicula Moranensis J
Pinguicula Gypsicola x Kohres
VFT DCXL (leaves)
VFT Red Piranha (leaves)
VFT Cupped trap (leaves)
VFT King Henry (traps)
Pinguicula Aphrodite
Pinguicula Yuca
Pinguicula Hemiepiphytica x Rotundifolia
Drosera Binata
Drosera Andromeda
VFT DCXL (traps)
VFT Red Piranha (traps)
VFT B52 (traps)
Drosera fluva
Drosera aff. Lanata
Drosera Gyamolenesis
Drosera Falconeri
Drosera Broomensis
Drosera Darwinensis
Sphagnum moss


Stay tuned for updates on the progress of this experiment! Are you using PPM in your Tissue Culture process? Then we want to hear from you! Send photos of your home Tissue Culture lab or updates on your experiments to zaire@plantcelltechnology.com for a chance to be featured!