Plant Preservative Mixture (PPM), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne and endogenous contamination.
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About Plant Preservative Mixture (PPM)
PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture.
- At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
- PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
- PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.
NOTE: 1L orders of PPM may arrive in two 500mL containers due to supply chain demands.
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Volume 1 Liter
I buy 1 Liter, but maybe customs officers in Indonesia took 250mL as sample, so I only get 750mL. Well, that's okey. I give 5 stars to seller.
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Contamination eliminate
Product is very good only thing we have to pay more cash to shipping and pl pack as small because my 1st order packing you with details pepper I didn't pay custom tax in Srilanka but this time your packing bag is big thwy claim more tax me. Kindly consider it since next time.I used actually very little for plant tc media I very much prefer your product quick service onlything shipping and custom cost.Thanks give opportunity to say my opinions.
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Contamination eliminate
Product is very good only thing we have to pay more cash to shipping and pl pack as small because my 1st order packing you with details pepper I didn't pay custom tax in Srilanka but this time your packing bag is big thwy claim more tax me. Kindly consider it since next time.I used actually very little for plant tc media I very much prefer your product quick service onlything shipping and custom cost.Thanks give opportunity to say my opinions.
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Positive results in emergency situation
I had to unexpectedly flask an orchid green seed capsule with no access to an autoclave, laminar flow cabinet or glove box, I used only rudimentary sterilizing techniques and PPM. No contamination after nearly four weeks. Amazing!
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Amazing
So just to test the ability of the PPM to resist microbial contaminants - I dosed two plates of media, one with and one without, with several drops of actively aerated compost tea. In 7 days the plate without PPM was entirely overrun with fungi and bacteria - the plate with PPM has had zero visible growth at 1ml/l. Every culture I have placed in PPM media - has had no problems with unwanted contamination. Some magic stuff here.
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PPM
I am very grateful to have found PPM. In the past I was averaging a 70% contamination rate. Since using PPM I'm holding steady at a 5% contamination rate.
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Works Perfectly!
I'm new to micropropagation. I was concerned about contaminated cultures. I've use PPM in the first several media batches and all have been contamination-free. For me, PPM is a required ingredient in a media formulation.
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Makes a difference in home culture lab
I am a beginner hobbyist in tissue culture, still experimenting to get reliable results. There is a notable improvement in contamination rates when using PPM. I recommend it and will continue to use it in my culture medium.
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PPM
Had fungal growth in my latest batch of water-germinated VFT seeds. After rinsing the seeds w/0.5% bleach and then plating it into MS+PPM, they germinated without any contaminants. PPM works.
