Plant Preservative Mixture (PPM), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne and endogenous contamination.
PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture.
Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.
Cuando comence los cultivos invitro en mi casa, tuve que buscar un agente protector que ayudara a evitar la contaminación del medio de cultivo, así fue como di con este maravilloso producto. Lo mejor es que no tienes que abrir tus frascos autoclavados para agregar bactericidas o fungicidas, PPM lo podía autoclavar con el medio y eso fue lo mejor. Soy técnico agrícola profesional y recomiendo totalmente el uso de PPM para el cultivo invitro de plantas.
We use this in several of our cultures and it works really well!
I have used PPM for over 6 years now with my lilium tissue culture media and I have had very little contamination with it's use in less than .5%.
I used 4ml on a starting media, and so far i havent gotten any issue. My first try, all my batch grew mold after a week. This time, its been a week and a half and i havent seen any mold in my tissue culture. Also, sjopping around, i find this to be more economical where others are quite pricy. This one does the job and more.would buy again
From my experience, PPM is superior to the conventional use of antibiotics and anti-fungal agents in culture media.
My contamination rate has fallen dramatically to about 1%. Before PPM I was getting up to 60% contamination.
I use PPM for disinfection of the dry orchid seeds before sowing on media containing PPM and I get good germination and very low contamination.
I have been doing orchid seed growing and replating for many years. The primary problem has consistently been contamination- even after stringent disinfection of the seeds with hypochlorite or Hydrogen Peroxide, sterilisation of the culture media in a pressure cooker and aseptic transfer techniques.
I have used antibiotics in combination with anti-fungal agents in my media with only limited success.
Since I discovered PPM in 2016, my contamination rate has fallen drastically!
I clean the seeds and get them free from extraneous matter then soak in PPM solution for at least 12 hour then sow aseptically on pre-sterilized media which was prepared with PPM at a concentration of 1ml per litre.
I now have about a 1% contamination rate.
This is the best antimicrobial agent that I have encountered and I will continue using it.
i would recommend this to anyone tissue culturing, as it will increase success rate
I placed my first order for PPM and it was easy. We're tax exempt and the exemption was applied quickly to my order. Shipping was quick and received when expected.
I use it in hemp tissue culture and it works great