Supreme TC Grade Agar

$19.99 USD

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Size 100g 500g 1kg 5kg 25kg

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For large bulk orders, contact sales@plantcelltechnology.com.

Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium.

The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.

The benefits of agar as tissue culture media

Easy to use
Acts as a solid support for the growth and development of explants
Allows the absorption of nutrients by the plant cells
Cost-effective

How To Use Agar

Follow the given steps to prepare 1 liter of tissue culture media using PCT’s Supreme TC Agar:

Take a beaker and add 800 ml of distilled water to it.
To the water add 4.54 grams of PCT’s MS Media (or alternative medium based on your plant), 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
Make up the volume of the media to 1000ml.
Adjust the pH of the media between 5.4-5.8.
Add 6-8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
Autoclave the medium for 20 minutes at 15 psi and 121℃.

Your media is ready!

Product Information

Product Name	Supreme TC Grade Agar
Synonyms	Gelose, Agar Agar Agar
CAS No.	9002-18-0
Capacity	Available in different weights of 100gm, 500gm, 1Kg, and 5Kg
Shape	Cylindrical container
Material	Powder
Color	Light beige
Solubility	Partially soluble in cold water and completely soluble in hot water
Gelling Strength	980 g/cm2

Shipping & Storage

Shelf Life	See expiry date on the bottle
Storage Temperature	20-22 ºC
Storage	Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from combustible substances.
HS Code	1302.31

Applications

Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.