Supreme TC Grade Agar
Supreme TC Grade Agar
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What is Agar?
Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium.
The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.
The Benefits of Agar
- Easy to use
- Acts as a solid support for the growth and development of explants
- Allows the absorption of nutrients by the plant cells
- Cost-effective
How To Use Agar?
Follow the given steps to prepare the tissue culture media using MS media:
- Take a beaker and add 800 ml of sterile distilled water to it.
- To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
- Add water to make 950 ml of the volume of the media.
- Adjust the pH of the media between 5.8-6.0.
- Add 8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
- Make up the volume of the media to 1000ml.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
Product Name | Supreme TC Grade Agar |
Synonyms | Gelose, Agar Agar Agar |
CAS No. | 9002-18-0 |
Shelf Life | See expiry date on the bottle |
Capacity | Available in different weights of 100gm, 500gm, 1Kg, and 5Kg |
Shape | Cylindrical container |
Material | Powder |
Color | Light beige |
Solubility | Partially soluble in cold water and completely soluble in hot water |
Storage | Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from combustible substances. |
HTS Code | 1302-31-00 |
Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.
What is Agar?
Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium.
The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.
The Benefits of Agar
- Easy to use
- Acts as a solid support for the growth and development of explants
- Allows the absorption of nutrients by the plant cells
- Cost-effective
How To Use Agar?
Follow the given steps to prepare the tissue culture media using MS media:
- Take a beaker and add 800 ml of sterile distilled water to it.
- To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
- Add water to make 950 ml of the volume of the media.
- Adjust the pH of the media between 5.8-6.0.
- Add 8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
- Make up the volume of the media to 1000ml.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
Product Name | Supreme TC Grade Agar |
Synonyms | Gelose, Agar Agar Agar |
CAS No. | 9002-18-0 |
Shelf Life | See expiry date on the bottle |
Capacity | Available in different weights of 100gm, 500gm, 1Kg, and 5Kg |
Shape | Cylindrical container |
Material | Powder |
Color | Light beige |
Solubility | Partially soluble in cold water and completely soluble in hot water |
Storage | Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from combustible substances. |
HTS Code | 1302-31-00 |
Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.
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