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DKW Medium w/ Vitamins

DKW Medium w/ Vitamins

$19.99 USD
$19.99 USD
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What is DKW Media?

DKW medium is also known as Driver and Kuniyuki Walnut medium. The media was originally developed for walnut micropropagation using nodal explant. Notably, DKW medium contains lower nitrogen and higher sulfate content, potentially influencing plant growth and development.

Instructions for Use:

  1. Dissolve 5.36gms of the medium in 600ml of distilled or deionized water at room temperature (15-30°C).
  2. Rinse media vial with small quantity of distilled water to remove traces of power.
  3. Add the desired heat stable supplements before autoclaving.
  4. Continue stirring until the powder has dissolved.Make up the final volume to 1000ml with distilled water.
  5. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50°C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15lbs (121°C for 15 minutes.
  6. Cool the autoclaved culture vessels containing medium to 45-50°C and aseptically add desired sterile heat-labile substrate.
  7. Mix well and aseptically dispense desired quantity of the media in sterile culture vessels.

NOTE: Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH.

  • Product Name: DKW Medium Basal Medium with Vitamins
  • Appearance: White to light tan with homogenous mixture of free flowing powder
  • Formula weight: 5.36 g/L
  • Solubility: Water (Appear as colorless to slight yellow solution, clear, complete)
  • pH (at 25°C): 4.8 ± 0.2
  • Storage and Shelf Life: Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8°C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8°C.

Media Compositions


**FORMULA WT. 5.36 GMS/LTR**

Multiple shoot induction

Callus-mediated shoot regeneration

Improved success rates for specific plant species (e.g., turmeric and walnut)

Used to tissue culture woody plants

What is DKW Media?

DKW medium is also known as Driver and Kuniyuki Walnut medium. The media was originally developed for walnut micropropagation using nodal explant. Notably, DKW medium contains lower nitrogen and higher sulfate content, potentially influencing plant growth and development.

Instructions for Use:

  1. Dissolve 5.36gms of the medium in 600ml of distilled or deionized water at room temperature (15-30°C).
  2. Rinse media vial with small quantity of distilled water to remove traces of power.
  3. Add the desired heat stable supplements before autoclaving.
  4. Continue stirring until the powder has dissolved.Make up the final volume to 1000ml with distilled water.
  5. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50°C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15lbs (121°C for 15 minutes.
  6. Cool the autoclaved culture vessels containing medium to 45-50°C and aseptically add desired sterile heat-labile substrate.
  7. Mix well and aseptically dispense desired quantity of the media in sterile culture vessels.

NOTE: Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH.

  • Product Name: DKW Medium Basal Medium with Vitamins
  • Appearance: White to light tan with homogenous mixture of free flowing powder
  • Formula weight: 5.36 g/L
  • Solubility: Water (Appear as colorless to slight yellow solution, clear, complete)
  • pH (at 25°C): 4.8 ± 0.2
  • Storage and Shelf Life: Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8°C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8°C.

Media Compositions


**FORMULA WT. 5.36 GMS/LTR**

Documents

Video Tutorial

Multiple shoot induction

Callus-mediated shoot regeneration

Improved success rates for specific plant species (e.g., turmeric and walnut)

Used to tissue culture woody plants


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