PPMTM - Plant Preservative Mixture.    


Info: Plant culture preservative and biocide

Product Number: PPM

Storage Conditions: 39ºF / 4º C

Product Description

Plant Preservation Mixture (PPMTM) is a robust broad-spectrum biocide formulated for use in plant tissue culture.  PPMTM targets bacteria and fungi in plant tissue culture growth media as well as contaminated tissue.  It affects key enzymes in the Krebs cycle and in the Electron Transport Chain.  Depending on the dose and the level of contamination, PPM is a biocidal component in plant culture medium.  In addition,  it may also function as a  bio-static compound as a preventative measure.  When diluted with plant growth media it is effective as a micro-biocide (i.e. bactericide and fungicide) against non-human health pathogenic organisms component of liquid or semi-solid plant culture media.

PPMTM is effective for most seed baring plants - angiosperm, as well as gymnosperm,- however, it is not recommended for use in ferns, mosses, algae and certain aquatic plants.  Optimization may be required to maximize potency.  While PPM is an excellent tool in the prevention and elimination of culture contamination it is not a substitute for aseptic laboratory techniques and appropriate air handling systems are recommended.

Composition  Proprietary (See MSDS for list of active ingredients)

Physical Properties

Physical form:  Solution

Appearance:  Clear, colorless to amber

pH: 3.8


Store vial at 4 C.

See label for expiration date

PPM may be added to media prior to sterilization or directly to sterile media prior to dispensation.

Auto-clavable  at 1.05 kg/cm2 (15 psi) and 121°C for 20 minutes. 

PPM should be added post-autoclave to media containing proteins, prior to dispensing to culture vessels.

PPM at final concentration (diluted in medium) is stable at ambient temperatures for up to 1 month. 


It is a violation of Federal Law to use this product in a manner inconsistent with its labeling. See MSDS for safety, handling, and disposal instructions.


The instructions listed below are for general use.  Optimization may be required.


PPM Quick Reference




Concentration (v/v)

0.05 – 0.2%

5.0% (see note)




4 – 12 hours



Plant tissue types vary, optimization is required.

For freshly isolated protoplast or callus use 0.05%

Higher concentrations may result in toxicity or uptake

In 3X MS salts

(use dry powder formulation from commercial companies)

Do not use in combination with other sterilization techniques.

Do not adjust pH.

Treated tissues should be placed directly into culture medium of choice containing 0.05% PPM.

May be added to antibiotic cocktail.



PATENT NO. 5,750,402 - The formulation of PPM in tissue culture media at certain concentrations and the use of PPM in tissue culture at certain concentrations to prevent or eliminate microbial contamination is protected by US patent No. 5,750,402. Patents have been issued in Canada, New Zealand, Australia, the European Community, Israel and other countries. It is also patent pending in many other countries of the world.


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Kodym A, Temsch E, Bunn E, Delpratt J. Ploidy stability of somatic embryo-derived plants in two ecological keystone sedge species (Lepidosperma laterale and L. concavum, Cyperaceae). Aust J Bot. 2012;60(5):396-404.

Peña-Ramírez YJ, et al. Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]. PCTOC. 2011;105(2):203-209.

Haddadi F, Adb Aziz M, Saleh G. Abd Rashid A, Kamaladini H. Micropropagation of Strawberry cv. Camarosa: Prolific Shoot Regeneration from In Vitro Shoot Tips Using Thidiazuron with N6-benzylamino-purine. HortScience. 2010;45(3):453-456.

Jimenez VM, Castillo J, Tavares E, Guevara E, Montiel M. In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. PCTOC. 2006;86:389–395.

Compton ME, Koch JM. Influence of Plant Preservative Mixture (PPM) on adventitous organogenesis in Melon, Petunia, and Tobacco. In Vitro Cell. Dev. Biol.- Plant. 2001;37:259-261